中文名
重组蛋白IL-12 Protein, Mouse, Tag free, CHO
英文名
IL-12 Protein, Mouse, Tag free, CHO
描述
IL-12 蛋白, Mouse (CHO)具有T细胞生长和IFN-γ的刺激属性。
蛋白编号
P43431(R23-A215) & P43432(M23-S335)
氨基酸序列
p35: RVIPVSGPAR CLSQSRNLLK TTDDMVKTAR EKLKHYSCTA EDIDHEDITR
DQTSTLKTCL PLELHKNESC LATRETSSTT RGSCLPPQKT SLMMTLCLGS IYEDLKMYQT
EFQAINAALQ NHNHQQIILD KGMLVAIDEL MQSLNHNGET LRQKPPVGEA DPYRVKMKLC
ILLHAFSTRV VTINRVMGYL SSA p40: MWELEKDVYV VEVDWTPDAP
GETVNLTCDT PEEDDITWTS DQRHGVIGSG KTLTITVKEF LDAGQYTCHK GGETLSHSHL
LLHKKENGIW STEILKNFKN KTFLKCEAPN YSGRFTCSWL VQRNMDLKFN IKSSSSSPDS
RAVTCGMASL SAEKVTLDQR DYEKYSVSCQ EDVTCPTAEE TLPIELALEA RQQNKYENYS
TSFFIRDIIK PDPPKNLQMK PLKNSQVEVS WEYPDSWSTP HSYFSLKFFV RIQRKKEKMK
ETEEGCNQKG AFLVEKTSTE VQCKGGNVCV QAQDRYYNSS CSKWACVPCR VRS
分子量
The protein has a predicted MW of 21.70 & 35.79 kDa. It migrates to 24-27 kDa and 42-53 kDa band in SDS-PAGE under reducing conditions due to the glycosylation.
内毒素含量
<1.0 EU per 1 µg of the protein by the LAL method.
活性
Measured by its binding ability in a functional ELISA. Immobilized Mouse IL-12RB2 at 2 μg/mL (100 μL/well) can bind Mouse IL-12. The ED50 for this effect is 100-350 ng/mL.
组分
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 7% trehalose.
复溶方法
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
储存
Stored at -20 to -80°C for 12 months as supplied from date of receipt; 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid repeated freeze-thaw cycles.
阿达玛斯 - 生命科学（Adamas-life®）自 2020 年成立以来，始终深耕高端生物试剂领域，致力于为生物医药、临床诊断、食品环境、基础科研等多行业提供全流程解决方案，是国内生命科学试剂行业的创新引领者。
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