中文名
重组蛋白IL-4 Protein, Human, Tag free, CHO
英文名
IL-4 Protein, Human, Tag free, CHO
描述
IL-4 蛋白, Human (CHO)是一种 CHO 细胞来源的有效的淋巴细胞生长因子,刺激 B 细胞增殖分化,参与炎症反应。
氨基酸序列
HKCDITLQEI IKTLNSLTEQ KTLCTELTVT DIFAASKNTT EKETFCRAAT VLRQFYSHHE
KDTRCLGATA QQFHRHKQLI RFLKRLDRNL WGLAGLNSCP VKEANQSTLE NFLERLKTIM
REKYSKCSS
分子量
The protein has a predicted MW of 14.96 kDa. It migrates to 18-21 kDa band in SDS-PAGE under reducing conditions due to the glycosylation.
内毒素含量
<0.2 EU per 1 µg of the protein by the LAL method.
活性
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is <0.25 ng/mL.
组分
Lyophilized from sterile PBS, pH 7.4, 7% trehalose.
复溶方法
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
储存
Stored at -20 to -80°C for 12 months as supplied from date of receipt; 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid repeated freeze-thaw cycles.
关键词
rHuIL-4;BSF-1;Binetrakin;Lymphocyte stimulatory factor 1;Pitrakinra
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