中文名
重组蛋白IL-2 Protein, Human, Tag Free, E.coli
英文名
IL-2 Protein, Human, Tag Free, E.coli
描述
Product Name Human IL - 2 Protein The recombinant Human IL - 2 Protein is Source expressed from E.coli with no tag . Accession P60568 Expression fragment A21 - T153 The protein has a predicted MW of 15.42 kDa. Molecular Weight It migrates to 15 kDa band in SDS - PAGE under reducing conditions. 0.0327 EU per 1 µg of the protein by the LAL Endotoxin Level method / rFC method。 Purity >95%, by SDS - PAGE under reducing conditions. Actlvity The ED50 as determined by the dose - dependant stimulation of the proliferation of CTLL - 2 was found to be < 5 ng/mL. Lyophilized from sterile 10 mM sodium citrate, Formulation pH 4.0, 7% trehalose. Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μ g/mL is Reconstitution recommended. Dissolve the lyophilized protein in distilled water。
氨基酸序列
APTSSSTKKT QLQLEHLLLD LQMILNGINN YKNPKLTRML TFKFYMPKKA TELKHLQCLE
EELKPLEEVL NLAQSKNFHL RPRDLISNIN VIVLELKGSE TTFMCEYADE TATIVEFLNR
WITFCQSIIS TLT
分子量
The protein has a predicted MW of 15.4 kDa. It migrates to 17 kDa band in SDS-PAGE under reducing conditions.
内毒素含量
<0.1 EU per 1 µg of the protein by the LAL method.
活性
Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 295. The ED50 for this effect is 0.0300-0.250 ng/mL.
组分
Lyophilized from sterile 10 mM sodium citrate, pH 4.0.
复溶方法
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
储存
Stored at -20 to -80°C for 12 months as supplied from date of receipt; 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid repeated freeze-thaw cycles.
关键词
rHuIL-2;IL2;T-cell Growth Factor;TCGF;Aldesleukin
阿达玛斯 - 生命科学（Adamas-life®）自 2020 年成立以来，始终深耕高端生物试剂领域，致力于为生物医药、临床诊断、食品环境、基础科研等多行业提供全流程解决方案，是国内生命科学试剂行业的创新引领者。
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