中文名
重组蛋白IL-18, Human, Tag Free, E. coli
英文名
IL-18, Human, Tag Free, E. coli
描述
Product Name Human IL - 18 Protein The recombinant Human IL - 18 Protein is Source expressed from E.coli with no tag . Accession Q14116 - 1 Expression fragment Y37 - D193 The protein has a predicted MW of 18.21 kDa. Molecular Weight It migrates to 18 kDa band in SDS - PAGE under reducing conditions. 0.0388 EU per 1 µg of the protein by the LAL Endotoxin Level method / rFC method. Purity >95%, by SDS - PAGE under reducing conditions. Actlvity Measured by its ability to induce IFN - gamma secretion by KG 1 human acute myelogenous - leukemia cells in the presence of TNF - alpha. The ED50 for this effect is typically 2 - 60 ng/mL. Lyophilized from sterile PBS, pH 7.4, 7% Formulation trehalose. Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μ g/mL is Reconstitution recommended. Dissolve the lyophilized protein in distilled water。
氨基酸序列
YFGKLESKLS VIRNLNDQVL FIDQGNRPLF EDMTDSDCRD NAPRTIFIIS MYKDSQPRGM
AVTISVKCEK ISTLSCENKI ISFKEMNPPD NIKDTKSDII FFQRSVPGHD NKMQFESSSY
EGYFLACEKE RDLFKLILKK EDELGDRSIM FTVQNED
分子量
The protein has a predicted MW of 18.2 kDa. It migrates to 19 kDa band in SDS-PAGE under reducing conditions.
内毒素含量
<0.1 EU per 1 µg of the protein by the LAL method.
活性
Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 1-10 ng/mL.
组分
Lyophilized from sterile 20 mM PB, 150 mM NaCl, pH 7.4.
复溶方法
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
储存
Stored at -20 to -80°C for 12 months as supplied from date of receipt; 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid repeated freeze-thaw cycles.
关键词
Interleukin-18;Iboctadekin;Interferon gamma-inducing factor;IFN-gamma-inducing factor;Interleukin-1 gamma;IL-1 gamma;GIF;IL-18;IL-1g;IL1F4;MGC12320;IL18
阿达玛斯 - 生命科学（Adamas-life®）自 2020 年成立以来，始终深耕高端生物试剂领域，致力于为生物医药、临床诊断、食品环境、基础科研等多行业提供全流程解决方案，是国内生命科学试剂行业的创新引领者。
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