精准检测新维度：荧光免疫分析试剂与技术创新方案
2025-11-19 · 产品方案 · 百灵威授权转载
免疫层析技术是一种结合了免疫技术和色谱层析技术的快速检测分析方法，在即时检测中应用极为广泛。 荧光免疫层析法是在免疫荧光技术、层析技术和激光激发荧光技术的基础上发展而来。荧光免疫分析将荧光标记技术与免疫学反应相结合，通过荧光信号的高灵敏度检测实现对目标物的精准定性与定量分析。该技术在疾病诊断、生物标志物发现、药物研发及生命科学研究等领域发挥着关键作用。
荧光免疫检测体系涵盖荧光标记物、固相载体、反应缓冲液及信号检测系统等关键组分。在荧光标记物层面，传统有机染料与新型纳米材料的协同发展极大提升了检测性能；在固相载体方面，经过特殊处理的微球和芯片为免疫反应提供了理想平台；在反应体系优化中，专用封闭剂和稀释液有效降低非特异性吸附，提高信噪比。
现代荧光免疫技术已形成包括流式细胞分析、组织化学染色、微阵列检测及均相免疫分析等在内的多元化技术平台。以流式细胞术为例，其卓越的多参数分析能力可同时检测细胞表面和内部的多个靶标，在免疫分型和肿瘤研究中不可或缺；而时间分辨荧光技术的应用，则通过消除背景干扰将检测灵敏度推向新高度。
百灵威提供荧光免疫分析相关的试剂、仪器耗材，助力相关研究。
荧光标记物
| 品名 | CAS | 货号 |
|---|---|---|
| Fluorescein 5(6)-isothiocyanate, 95%, for fluorescence, mixture of 5- and 6- isomers 5(6)-异硫氰酸荧光素 | 27072-45-3 | 194222 |
| 6-FITC, 95% 6-异硫氰酸荧光素 | 3326-31-6 | 960900 |
| Fluorescein isothiocyanate isomer I, 95% 异硫氰酸荧光素异构体 I | 3326-32-7 | 255493 |
| Tetramethylrhodamine-5-(and-6)-isothiocyanate, 95%, suitable for fluorescence, mixture of isomers 四甲基罗丹明-5(6)-异硫氰酸 | 95197-95-8 | 953201 |
| Tetramethylrhodamine-5-isothiocyanate, 90% 四甲基罗丹明-5-异硫氰酸 | 80724-19-2 | 145736 |
| Green fluorescent microspheres, particle size : 200nm, 1%w/v, suspended in deionized water 绿色荧光微球 | / | 9435499 |
| Yellow fluorescent microspheres, λex:465nm, λem:510nm, 200nm, 1%w/v, suspended in deionized water 黄色荧光微球 | / | 9435567 |
| Orange fluorescent microspheres, λex:465nm, λem:585nm, 200nm, 1%w/v, suspended in deionized water 橙色荧光微球 | / | 9435570 |
| Red fluorescent microspheres, λex:535nm, λem:580nm, 200nm, 1%w/v, suspended in deionized water 红色荧光微球 | / | 9435573 |
| Purple fluorescent microspheres, λex:545nm, λem:615nm, 300nm, 1%w/v, suspended in deionized water 紫色荧光微球 | / | 9435577 |
| Time-resolved fluorescent microspheres, 200nm, 1%w/v, suspended in deionized water 时间分辨荧光微球 | / | 9435497 |
| Time-resolved fluorescent microspheres, 300nm, 1%w/v, suspended in deionized water 时间分辨荧光微球 | / | 9435498 |
| Ultra high sensitivity time-resolved fluorescent microspheres, 100nm, 1% w/v, functional group: carboxyl, suspended in deionized water 超高灵敏度时间分辨荧光微球 | / | 9435540 |
| Ultra high sensitivity time-resolved fluorescent microspheres, 200nm, 1%w/v, functional group: carboxyl, suspended in deionized water 超高灵敏度时间分辨荧光微球 | / | 9435541 |
| Time-resolved fluorescent microspheres, 100nm, 1%w/v, functional group: carboxyl, suspended in deionized water 时间分辨荧光微球 | / | 9435544 |
| Visualized time-resolved fluorescent microspheres, 200nm, 1%w/v, functional group: carboxyl, suspended in deionized water 可视化时间分辨荧光微球 | / | 9435546 |
| Gold nanorods modified with PEG, 0.1 mg/mL in ultrapure water, surface group: —PEG—NH2, λ=660±15nm PEG化金纳米棒 | 7440-57-5 | 936779 |
| Oil soluble silver nanoparticles, 0.1 mg/mL; Solvent: Xylene; Particle size: 100 ± 5 nm; Surface functional groups: aromatic hydrocarbons 油溶性银纳米颗粒 | / | 9229077 |
固相载体
| 品名 | CAS | 货号 |
|---|---|---|
| Amino Fe3O4 magnetic beads , 0.1 - 0.2 μm, 0.5% (w/v) suspended in PBS buffer solution 氨基四氧化三铁磁珠 | 1317-61-9 | 1924547 |
| Amino polystyrene magnetic beads, 1 - 2 μm, 0.5% (w/v) suspended in PBS buffer solution 氨基聚苯乙烯磁珠 | / | 1924551 |
| Amino silica magnetic beads, SLC, 1 - 2 μm, 1% (w/v) suspended in PBS buffer solution 氨基二氧化硅磁珠 | 14808-60-7 | 952697 |
| Carboxylated Fe3O4 magnetic beads, 0.1 - 0.2 μm, 0.5% (w/v) suspended in PBS buffer solution 羧基四氧化三铁磁珠 | 1317-61-9 | 1924549 |
| Carboxylated polystyrene magnetic beads, 1 - 2 μm, 0.5% (w/v) suspended in PBS buffer solution 羧基聚苯乙烯磁珠 | / | 1924552 |
| Carboxylated silica magnetic beads, SLC, 1 - 2 μm, 1% (w/v) suspended in PBS buffer solution 羧基二氧化硅磁珠 | 14808-60-7 | 982842 |
| Hydroxy Fe3O4 magnetic beads, 0.1 - 0.2 μm, 0.5% (w/v) suspended in PBS buffer solution 羟基四氧化三铁磁珠 | 1317-61-9 | 1924550 |
| Silicon hydroxyl group polystyrene magnetic beads, 1 - 2 μm, 0.5% (w/v) suspended in PBS buffer solution 硅羟基聚苯乙烯磁珠 | / | 1924553 |
反应缓冲液与辅助试剂
| 品名 | CAS | 货号 |
|---|---|---|
| Albumin from human serum, 96% 人血清白蛋白 | 70024-90-7 | 601386 |
| Bovine serum albumin, 98%, from bovine serum 牛血清白蛋白 | 9048-46-8 | 900523 |
| Casein, 92% protein 酪蛋白 | 9000-71-9 | 166199 |
| White blood cell dilution solution 白细胞稀释液 | / | 9456078 |
仪器耗材
| 品名 | CAS | 货号 |
|---|---|---|
| Single-channel mechanical pipettor, Volume range: 0.5 - 10 μL, Increment: 0.1 μL 单道手动可调移液器 | / | 1122421 |
| Centrifuge tubes, 15 mL, conical bottom, sterile, 100 pcs/pack 离心管 | / | 9116437 |
| PCR tubes, 0.2 mL, individual, attached flat cap,fluorescent quantitation, 500 tubes/pack PCR单管 | / | 1438746 |
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免疫诊断技术及相关原辅料
