杨有军教授科研成果 | EC系列近红外荧光染料——荧光强度高，光稳定性好
2024-01-18 · 系列介绍 · 百灵威授权转载
EC系列近红外荧光染料是华东理工大学杨有军教授团队开发的一类新型高性能荧光染料（图1）[1-7]，满足生物医学领域对红外成像、光热消融等应用需求。利用自主搭建的荧光显微成像设备，可对细胞或活体进行荧光成像。
![图1 EC dyes<sup>[1]</sup>](/assets/jk-articles-all/fb6ca08685fdf5b3ea1ff8a0.png)
图1 EC dyes[1]
与传统花菁类近红外荧光染料（如Cy5、吲哚菁绿）相比，EC系列近红外荧光染料在吸收发射波长、荧光强度、光稳定性、环境敏感性等关键指标方面有明显优势：
- 谱峰窄，荧光强度高：吸收发射光谱的谱峰窄（FWHM=45 nm），荧光强度高（ε×φ>40,000 M-1cm-1），荧光标记、成像效果好；
- 光稳定性好：EC荧光染料在相同时间内，光漂白比例仅为吲哚菁绿的1/10-1/1000；
- 具生物相容性：λex＜800 nm的EC荧光染料，具有良好的细胞透膜性，便于细胞或者生物组织染色。
根据用途有如下分类：
适用于细胞水平的荧光显微成像
- 具有优异的发光性质，抗光漂白、抗氧化、抗生物硫醇加成性质。
- 具有跨细胞膜能力，定位在细胞线粒体。
| EC荧光染料 | Fluorescence | Molar Extinction Coefficient |
|---|---|---|
| EC640, 97%, Near-infrared fluorescent dye, liposoluble EC640, 97%, 脂溶性近红外荧光染料 | λex 640 nm; λem 659 nm | >130,000 cm -1M-1 |
| EC710, 97%, Near-infrared fluorescent dye, water-soluble EC710, 97%, 水溶性近红外荧光染料 | λex 712 nm; λem 740 nm | > 59,100 cm -1M-1 |
适用于细胞及小动物活体荧光显微成像
- 具有优异的发光性质，抗光漂白、抗氧化、抗生物硫醇加成性质。
- 不能跨细胞膜。
- 同时还具有光热、光声、光动力性质。
| EC荧光染料 | Fluorescence | Molar Extinction Coefficient |
|---|---|---|
| EC825, 97%, Near-infrared fluorescent dye, liposoluble EC825, 97%, 脂溶性近红外荧光染料 | λex 825 nm; λem 857 nm | >269,000 cm -1M-1 |
| EC835, 97%, Near-infrared fluorescent dye, liposoluble EC835, 97%, 脂溶性近红外荧光染料 | λex 835 nm; λem 870 nm | >226,000 cm -1M-1 |
| ESi860, 97%, Near-infrared fluorescent dye, water-soluble ESi860, 97%, 水溶性近红外荧光染料 | λex 862 nm; λem 895 nm | >137,960 cm -1M-1 |
| EC870, 97%, Near-infrared fluorescent dye, water-soluble EC870, 97%, 水溶性近红外荧光染料 | λex 835 nm; λem 923 nm | >185,200 cm -1M-1 |
| ESi880, Near-infrared fluorescent dye, water-soluble ESi880, 水溶性近红外荧光染料 | λex 877 nm; λem 916 nm | >180,520 cm -1M-1 |
适用于小动物活体荧光显微成像
- 具有优异的发光性质，抗光漂白、抗氧化、抗生物硫醇加成性质。不能跨细胞膜。同时还具有光热、光声性质。
- 其中EC900荧光染料光动力性质弱，Ka<0，适用于极酸性体系。
- EC1200荧光染料无光动力性质。
| EC荧光染料 | Fluorescence | Molar Extinction Coefficient |
|---|---|---|
| EC900, 97%, Near-infrared fluorescent dye, water-soluble EC900, 97%, 水溶性近红外荧光染料 | λex 901 nm; λem 976 nm | >163,000 cm -1M-1 |
| EC1200, 97%, Near-infrared fluorescent dye, liposoluble EC1200, 97%, 脂溶性近红外荧光染料 | λex 1208 nm; λem 1280 nm | >391,000 cm -1M-1 |
产品列表
EC640
| 品名 | 特性 | 货号 |
|---|---|---|
| EC640-Br, 97%, Near-infrared fluorescent dye, liposoluble EC640-Br, 97%, 脂溶性近红外荧光染料 | 与O/S/N等 亲核试剂反应 | 9372065 |
| EC640-N3, 97%, Near-infrared fluorescent dye, liposoluble EC640-N3, 97%, 脂溶性近红外荧光染料 | 与端基炔通过 点击化学偶联 | 9372066 |
| EC640WS, 97%, Near-infrared fluorescent dye, water-soluble EC640WS, 97%, 水溶性近红外荧光染料 | 水溶性 | 9189157 |
| EC640-WSN3, 97%, Near-infrared fluorescent dye, water-soluble EC640-WSN3, 97%, 水溶性近红外荧光染料 | 水溶性，炔基点击偶联 | 9372068 |
EC710
| 品名 | 特性 | 货号 |
|---|---|---|
| EC710-Br, 97%, Near-infrared fluorescent dye, water-soluble EC710-Br, 97%, 水溶性近红外荧光染料 | 与O/S/N等 亲核试剂反应 | 9372098 |
| EC710-N3, 97%, Near-infrared fluorescent dye, water-soluble EC710-N3, 97%, 水溶性近红外荧光染料 | 与端基炔通过 点击化学偶联 | 9372099 |
| EC710-WS, 97%, Near-infrared fluorescent dye, water-soluble EC710-WS, 97%, 水溶性近红外荧光染料 | 水溶性 | 9372100 |
| EC710-WSN3, 97%, Near-infrared fluorescent dye, water-soluble EC710-WSN3, 97%, 水溶性近红外荧光染料 | 水溶性，炔基点击偶联 | 9372101 |
EC825
| 品名 | 特性 | 货号 |
|---|---|---|
| EC825-Br, 97%, Near-infrared fluorescent dye, liposoluble EC825-Br, 97%, 脂溶性近红外荧光染料 | 与O/S/N等 亲核试剂反应 | 9372069 |
| EC825-N3, 97%, Near-infrared fluorescent dye, liposoluble EC825-N3, 97%, 脂溶性近红外荧光染料 | 与端基炔通过 点击化学偶联 | 9372070 |
| EC825WS, 97%, Near-infrared fluorescent dye, water-soluble EC825WS, 97%, 水溶性近红外荧光染料 | 水溶性 | 9189161 |
| EC825-WSN3, 97%, Near-infrared fluorescent dye, water-soluble EC825-WSN3, 97%, 水溶性近红外荧光染料 | 水溶性，炔基点击偶联 | 9372072 |
EC835
| 品名 | 特性 | 货号 |
|---|---|---|
| EC835-Br, 97%, Near-infrared fluorescent dye, liposoluble EC835-Br, 97%, 脂溶性近红外荧光染料 | 与O/S/N等 亲核试剂反应 | 9372074 |
| EC835-N3, 97%, Near-infrared fluorescent dye, liposoluble EC835-N3, 97%, 脂溶性近红外荧光染料 | 与端基炔通过 点击化学偶联 | 9372075 |
| EC835WS, 97%, Near-infrared fluorescent dye, water-soluble EC835WS, 97%, 水溶性近红外荧光染料 | 水溶性 | 9189162 |
| EC835-WSN3, 97%, Near-infrared fluorescent dye, water-soluble EC835-WSN3, 97%, 水溶性近红外荧光染料 | 水溶性，炔基点击偶联 | 9372077 |
ESi860
| 品名 | 特性 | 货号 |
|---|---|---|
| ESi860-Br, 97%, Near-infrared fluorescent dye, water-soluble ESi860-Br, 97%, 水溶性近红外荧光染料 | 与O/S/N等 亲核试剂反应 | 9372079 |
| ESi860-N3, 97%, Near-infrared fluorescent dye, water-soluble ESi860-N3, 97%, 水溶性近红外荧光染料 | 与端基炔通过 点击化学偶联 | 9372080 |
| ESi860-WS, 97%, Near-infrared fluorescent dye, water-soluble ESi860-WS, 97%, 水溶性近红外荧光染料 | 水溶性 | 9372081 |
EC870
| 品名 | 特性 | 货号 |
|---|---|---|
| EC870-Br, 97%, Near-infrared fluorescent dye, water-soluble EC870-Br, 97%, 水溶性近红外荧光染料 | 与O/S/N等 亲核试剂反应 | 9372083 |
| EC870-WS, 97%, Near-infrared fluorescent dye, water-soluble EC870-WS, 97%, 水溶性近红外荧光染料 | 水溶性 | 9372084 |
| EC870-WSN3, 97%, Near-infrared fluorescent dye, water-soluble EC870-WSN3, 97%, 水溶性近红外荧光染料 | 水溶性，炔基点击偶联 | 9372085 |
ESi880
| 品名 | 特性 | 货号 |
|---|---|---|
| ESi880-N3, 97%, Near-infrared fluorescent dye, water-soluble ESi880-N3, 97%, 水溶性近红外荧光染料 | 与O/S/N等 亲核试剂反应 | 9372086 |
| ESi880-WS, 97%, Near-infrared fluorescent dye, water-soluble ESi880-WS, 97%, 水溶性近红外荧光染料 | 水溶性 | 9372087 |
| ESi880-WSN3, 97%, Near-infrared fluorescent dye, water-soluble ESi880-WSN3, 97%, 水溶性近红外荧光染料 | 水溶性，炔基点击偶联 | 9372088 |
EC900
| 品名 | 特性 | 货号 |
|---|---|---|
| EC900-Br, 97%, Near-infrared fluorescent dye, water-soluble EC900-Br, 97%, 水溶性近红外荧光染料 | 与O/S/N等 亲核试剂反应 | 9372090 |
| EC900-N3, 97%, Near-infrared fluorescent dye, water-soluble EC900-N3, 97%, 水溶性近红外荧光染料 | 与端基炔通过 点击化学偶联 | 9372091 |
| EC900-WS, 97%, Near-infrared fluorescent dye, water-soluble EC900-WS, 97%, 水溶性近红外荧光染料 | 水溶性 | 9372092 |
| EC900-WSN3, 97%, Near-infrared fluorescent dye, water-soluble EC900-WSN3, 97%, 水溶性近红外荧光染料 | 水溶性，炔基点击偶联 | 9372093 |
EC1200
| 品名 | 特性 | 货号 |
|---|---|---|
| EC1200-Br, 97%, Near-infrared fluorescent dye, liposoluble EC1200-Br, 97%, 脂溶性近红外荧光染料 | 与O/S/N等 亲核试剂反应 | 9372094 |
| EC1200-N3, 97%, Near-infrared fluorescent dye, liposoluble EC1200-N3, 97%, 脂溶性近红外荧光染料 | 与端基炔通过 点击化学偶联 | 9372095 |
| EC1200WS, 97%, Near-infrared fluorescent dye, water-soluble EC1200WS, 97%, 水溶性近红外荧光染料 | 水溶性 | 9189165 |
参考文献
- Lei Z, Li X, Luo X,et al. Bright, Stable, and Biocompatible Organic Fluorophores Absorbing/Emitting in the Deep Near-Infrared Spectral Region. Angewandte Chemie, 2017, 129(11):3025-3029.
- Jin Li, Yan Dong, Ruwei Wei, et al. Stable, Bright, and Long-Fluorescence-Lifetime Dyes for Deep-Near-Infrared Bioimaging. J. Am. Chem. Soc. 2022, 144, 14351–14362.
- Ruwei Wei, Yan Dong, Xueli Wang, et al. Rigid and Photostable Shortwave Infrared Fluorophore (EC7) Absorbing/Emitting beyond 1200 nm for High-Contrast Multiplexed Imaging. J. Am. Chem. Soc., 2023, 145, 12013–12022.
- Xicun Lu, Xiaoli Zhuang, Yan Dong, et al. Super-Photostability and Super-Brightness of EC5 Dyes for Super-Resolution Microscopy in the Deep Near-Infrared Spectral Region. Chem. Mater. 2024, 36, 949–958.
- Xicun Lu, Xiaoli Zhuang, Lei Yin, et al. Highly Photostable Deep-NIR Chromophore with a Superior Molar Absorptivity for Efficient Photothermal Therapy. Ind. Eng. Chem. Res. 2024, 63, 1239–1247.
- Yan Dong, Xicun Lu, Yi Li, et al. Spectral and biodistributional engineering of deep near-infrared chromophore. Chin. Chem. Lett., 2023, 34, 108154.
- Yan Dong, Ye Zou, Xiaotong Jia,et al. An acidic medium-compatible deep-near- infrared dye for in vivo imaging. Smart Molecules 2023, 1, e20230001.
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