张平玉教授科研成果｜人血清白蛋白检测探针——HSA-Ir
2023-11-17 · 推荐产品 · 百灵威授权转载
人血清白蛋白(HSA)是人血浆中的蛋白质。血液或泌尿系统中的HSA浓度是各种疾病如肾脏疾病、低蛋白血症等的可靠指标。
尿液中存在过量的HSA可导致微量白蛋白尿，是心血管疾病和肾脏的早期标志物。另一方面，血浆中HSA水平低会导致低蛋白血症，是肝硬化和慢性肝炎的症状。
因此，HSA的选择性检测是生化科研和临床研究的需求，在蛋白质组学研究、诊断学、治疗学等方面具有重要意义。
深圳大学张平玉教授研发出一种金属铱配合物——HSA-Ir荧光探针（图1），对不同浓度的HSA有明显的荧光响应[1]。
![图1 X-ray crystal structure of HSA-Ir Probe <sup>[1]</sup>.](/assets/jk-articles-all/55295f047a5ab2bebdbdcf58.png)
图1 X-ray crystal structure of HSA-Ir Probe [1].
- 染色比传统的考马斯亮蓝效果好（图1）；
- 染色过程更简单且耗时更短（染色时间 2min）；
- HSA检测限度低至1μl。
![图2 HSA-Ir Probe和考马斯亮蓝染色效果对比图 <sup>[1]</sup>.(a) The gel of different concentrations of HSA treated with Coomassie blue G250 (100 mg L1 , 1 h) or Ir1 (5 mM, 2 min) after separation. (b) Selective staining: the gel of HSA, blood serum and mixture of other proteins (hemoglobin, transferrin, myoglobin, g-globulin, hemin, lysozyme, apo-transferrin, methemoglobin and holo-transferrin) was stained with Ir1. Lanes A and D: mixture of other proteins; lane B: HSA; lane C: HSA + mixture of other proteins; lane E: blood serum; lane F: blood serum + mixture with other proteins. (c) The gel of HSA (10 mg) per lane and then treated with Ir1 for different incubation times after separation. Twice in parallel with each experiment. (d) The gel of different concentrations of HSA and then treated with Ir1 after separation.](/assets/jk-articles-all/e5af9fd96641cf66de1871ba.gif)
图2 HSA-Ir Probe和考马斯亮蓝染色效果对比图 [1].(a) The gel of different concentrations of HSA treated with Coomassie blue G250 (100 mg L1 , 1 h) or Ir1 (5 mM, 2 min) after separation. (b) Selective staining: the gel of HSA, blood serum and mixture of other proteins (hemoglobin, transferrin, myoglobin, g-globulin, hemin, lysozyme, apo-transferrin, methemoglobin and holo-transferrin) was stained with Ir1. Lanes A and D: mixture of other proteins; lane B: HSA; lane C: HSA + mixture of other proteins; lane E: blood serum; lane F: blood serum + mixture with other proteins. (c) The gel of HSA (10 mg) per lane and then treated with Ir1 for different incubation times after separation. Twice in parallel with each experiment. (d) The gel of different concentrations of HSA and then treated with Ir1 after separation.
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参考文献
- Yi Wang, Huaiyi Huang, Ge Chen, Haijie Chen, Tingting Xu, Qian Tang, Hailiang Zhu, Qianling Zhang and Pingyu Zhang, Chem. Commun., 2018, 54, 3282—3285.
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