分析实验室常备“武器”之HPLC衍生化试剂
2020-10-19 · 系列介绍 · 百灵威授权转载
高效液相色谱（HPLC）是现代化合物分离鉴定的重要手段，然而，由于缺少通用检测器、样品被测组分含量低及其本身性质的限制，并非所有化合物都可直接用于高效液相色谱分析。衍生化反应可改变被测化合物性质，使之能够快速、灵敏、准确地被测定。
百灵威提供紫外、荧光等多种HPLC衍生化试剂。
常用紫外衍生化试剂
高效液相色谱大都配备紫外检测器，为使一些没有或有很弱紫外吸收的化合物能被检测，往往需要通过衍生化反应，在这些化合物分子中引入具有强紫外吸收的基团，使衍生物可被紫外检测器检测。
A5506
N,N'-Diisopropyl-O-(4-nitrobenzyl)isourea, 95.0%(LC) 2978-11-2
N,N'-二异丙基-O-(4-硝基苯甲基)异脲
λmax: 254nm
A5523
Nalpha-(5-Fluoro-2,4-dinitrophenyl)-L-leucinamide, 98.0%(LC)178065-29-7
Nα-(5-氟-2,4-二硝基苯基)-L-亮氨酰胺
λmax: 340nm
A5524
Nalpha-(5-Fluoro-2,4-dinitrophenyl)-D-leucinamide, 98.0%(LC)178065-30-0
Nα-(5-氟-2,4-二硝基苯基)-D-亮氨酰胺
λmax: 340nm
368359
N-(4-Nitrophenylacetoxy)succinimide, 98%, derivatization grade, for HPLC Labeling68123-33-1
N-(4-硝基苯基乙酰氧基)琥珀酰亚铵
λmax: 254nm
210054
2,3,4,6-Tetra-O-acetyl-β-D-glucopyranosyl isothiocyanate, 98%14152-97-7
2,3,4,6-四-O-乙酰基-β-D-葡萄糖异硫氰酸酯
λmax: 254nm
563716
2,3,4,6-Tetra-O-benzoyl-β-D-glucopyranosyl isothiocyanate132413-50-4
2,3,4,6-四-O-苯甲酰基-β-D-吡喃葡萄糖基异硫氰酸酯
λmax: 254nm
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常用荧光衍生化试剂
荧光检测器具有高灵敏度和高选择性，比紫外检测器的灵敏度高10‐1000倍。荧光衍生化试剂能与一些官能团产生强烈荧光，在较温和条件下与被测物质快速定量反应，尤其适合痕量组分分析。
A5554
4-Nitro-7-piperazino-2,1,3-benzoxadiazole, 98.0%(LC&T)139332-66-4
NBD-PZ, 4-硝基-7-哌嗪-2,1,3-苯并恶二唑
λex: 470 nm; λem: 541 nm
228839
4-Bromomethyl-6,7-dimethoxycoumarin, 98%88404-25-5
4-溴甲基-6,7-二甲氧基香豆素
λex: 340 nm; λem: 425 nm
A5553
3-Bromomethyl-7-methoxy-1,4-benzoxazin-2-one, 98.0%(T)124522-09-4
3-溴甲基-7-甲氧基-1,4-苯并恶嗪-2-酮
λex: 355 nm; λem: 430 nm
A5560
(S)-(+)-4-(N,N-Dimethylaminosulfonyl)-7-(3-aminopyrrolidin-1-yl)-2,1,3-benzoxadiazole, HPLC Labeling Reagent for e.e. Determination143112-50-9
(S)-(+)-DBD-Apy, (S)-(+)-4-(N,N-二甲氨基磺酰)-7-(3-氨基吡咯烷-1-基)-2,1,3-苯并恶二唑
λex: 470 nm; λem: 580 nm
A5561
(R)-(-)-4-(N,N-Dimethylaminosulfonyl)-7-(3-aminopyrrolidin-1-yl)-2,1,3-benzoxadiazole, HPLC Labeling Reagent for e.e. Determination143112-49-6
(R)-(-)-DBD-Apy, (R)-(-)-4-(N,N-二甲基氨基磺酰基)-7-(3-氨基吡咯烷-1-基)-2,1,3-苯并恶二唑
λex: 470 nm; λem: 580 nm
A5562
(S)-(+)-4-Nitro-7-(3-aminopyrrolidin-1-yl)-2,1,3-benzoxadiazole, HPLC Labeling Reagent for e.e. Determination143112-52-1
(S)-(+)-NBD-Apy, (S)-(+)-4-硝基-7-(3-氨基吡咯烷-1-基)-2,1,3-苯并恶二唑
λex: 470 nm; λem: 540 nm
A5563
(R)-(-)-4-Nitro-7-(3-aminopyrrolidin-1-yl)-2,1,3-benzoxadiazole, HPLC Labeling Reagent for e.e. Determination143112-51-0
(R)-(-)-NBD-Apy, (R)-(-)-4-硝基-7-(3-氨基吡咯烷-1-基)-2,1,3-苯并恶二唑
λex: 470 nm; λem: 540 nm
A5555
4-(N,N-Dimethylaminosulfonyl)-7-piperazino-2,1,3-benzoxadiazole, for HPLC Labeling139332-64-2
DBD-PZ, 4-(N,N-二甲氨基磺酰基)-7-哌嗪-2,1,3-苯并恶二唑
λex: 440 nm; λem: 569 nm
A5573
4-(N-Hydrazinocarbonylmethyl-N-methylamino)-7-nitro-2,1,3-benzoxadiazole, 95.0%(LC)221263-97-4
NBD-CO-Hz, 4-(N-肼羰甲基-N-甲氨基)-7-硝基-2,1,3-苯并恶二唑
λex: 475 nm; λem: 530 nm
A5574
4-(N,N-Dimethylaminosulfonyl)-7-(2-aminoethylamino)-2,1,3-benzoxadiazole189373-41-9
DBD-ED, 4-(N,N-二甲氨基磺酰)-7-(2-氨基乙基氨基)-2,1,3-苯并恶二唑
λex: 450 nm; λem: 560 nm
A5576
4-Acetamido-7-mercapto-2,1,3-benzoxadiazole, 95.0%(LC)254973-02-9
AABD-SH, 4-乙酰氨基-7-巯基-2,1,3-苯并恶二唑
λex: 368 nm; λem: 524 nm
A5558
4-(N,N-Dimethylaminosulfonyl)-7-(N-chloroformylmethyl-N-methylamino)-2,1,3-benzoxadiazole, 93.0%(T)156153-43-4
DBD-COCl, 4-(N,N-二甲基氨磺酰)-7-(N-氯甲酰甲基-N-甲氨基)-2,1,3-苯并恶二唑
λex: 450 nm; λem: 560 nm
A5595
4-(N,N-Dimethylaminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole, 98.0%(LC)98358-90-8
DBD-F, 4-(N,N-二甲氨基磺酰)-7-氟-2,1,3-苯并恶二唑
λex: 450 nm; λem: 590 nm
A5569
(S)-(+)-4-(N,N-Dimethylaminosulfonyl)-7-(3-isothiocyanatopyrrolidin-1-yl)-2,1,3-benzoxadiazole, for HPLC Labeling163927-32-0
(S)-(+)-DBD-Py-NCS, (S)-(+)-4-(N,N-二甲氨基磺酰基)-7-(3-异硫氰酸基四氢吡咯-1-基)-2,1,3-苯并恶二唑
λex: 460 nm; λem: 550 nm
A5565
(R)-(+)-4-(N,N-Dimethylaminosulfonyl)-7-(2-chloroformylpyrrolidin-1-yl)-2,1,3-benzoxadiazole, HPLC Labeling Reagent for e.e. Determination150993-62-7
(R)-(+)-DBD-Pro-COCl, (R)-(+)-4-(N,N-二甲氨基磺酰基)-7-(2-氯甲酰四氢吡咯-1-基)-2,1,3-苯并恶二唑
λex: 450 nm; λem: 560 nm
A5564
(S)-(-)-4-(N,N-Dimethylaminosulfonyl)-7-(2-chloroformylpyrrolidin-1-yl)-2,1,3-benzoxadiazole, HPLC Labeling Reagent for e.e. Determination150993-63-8
(S)-(-)-DBD-Pro-COCl, (S)-(-)-4-(N,N-二甲氨基磺酰基)-7-(2-氯甲酰四氢吡咯-1-基)-2,1,3-苯并恶二唑
λex: 450 nm; λem: 560 nm
A5568
(R)-(-)-4-(N,N-Dimethylaminosulfonyl)-7-(3-isothiocyanatopyrrolidin-1-yl)-2,1,3-benzoxadiazole, for HPLC Labeling163927-31-9
(R)-(-)-DBD-Py-NCS, (R)-(-)-4-(N,N-二甲氨基磺酰基)-7-(3-异硫氰酸基四氢吡咯-1-基)-2,1,3-苯并恶二唑
λex: 460 nm; λem: 550 nm
A5579
4-(4,5-Diphenyl-1H-imidazol-2-yl)benzoyl Chloride Hydrochloride, 97.0%(T)N/A
4-(4,5-二苯基-1H-咪唑-2-基)苯甲酰氯盐酸盐
λex: 325 nm; λem: 430 nm
A5575
4-(N,N-Dimethylaminosulfonyl)-7-isothiocyanato-2,1,3-benzoxadiazole147611-81-2
DBD-NCS, 4-(N,N-二甲基氨磺酰)-7-异硫氰酸基-2,1,3-苯并恶二唑
λex: 384 nm; λem: 520 nm
A5592
4-Chloro-7-nitro-2,1,3-benzoxadiazole, 98.0%(T)10199-89-0
NBD-Cl, 4-氯-7-硝基-2,1,3-苯并恶二唑
λex: 460 nm; λem: 535 nm
A5566
(R)-(+)-4-Nitro-7-(2-chloroformylpyrrolidin-1-yl)-2,1,3-benzoxadiazole, HPLC Labeling Reagent for e.e. Determination159717-69-8
(R)-(+)-NBD-Pro-COCl, (R)-(+)-4-硝基-7-(2-氯甲酰四氢吡咯-1-基)-2,1,3-苯并恶二唑
λex: 470 nm; λem: 540 nm
A5567
(S)-(-)-4-Nitro-7-(2-chloroformylpyrrolidin-1-yl)-2,1,3-benzoxadiazole, HPLC Labeling Reagent for e.e. Determination159717-68-7
(S)-(-)-NBD-Pro-COCl, (S)-(-)-4-硝基-7-(2-氯甲酰四氢吡咯-1-基)-2,1,3-苯并恶二唑
λex: 470 nm; λem: 540 nm
A5577
(R)-(-)-4-(3-Isothiocyanatopyrrolidin-1-yl)-7-nitro-2,1,3-benzoxadiazole, HPLC Labeling Reagent for e.e. Determination163927-29-5
(R)-(-)-NBD-Py-NCS, (R)-(-)-4-(3-异硫氰酸基吡咯烷-1-基)-7-硝基-2,1,3-苯并恶二唑]
λex: 488 nm; λem: 590 nm
A5578
(S)-(+)-4-(3-Isothiocyanatopyrrolidin-1-yl)-7-nitro-2,1,3-benzoxadiazole, HPLC Labeling Reagent for e.e. Determination163927-30-8
(S)-(+)-NBD-Py-NCS, (S)-(+)-4-(3-异硫氰酸基吡咯烷-1-基)-7-硝基-2,1,3-苯并恶二唑
λex: 488 nm; λem: 590 nm
A5558
4-(N,N-Dimethylaminosulfonyl)-7-(N-chloroformylmethyl-N-methylamino)-2,1,3-benzoxadiazole, for HPLC Labeling156153-43-4
DBD-COCl, 4-(N,N-二甲基氨磺酰)-7-(N-氯甲酰甲基-N-甲氨基)-2,1,3-苯并恶二唑
λex: 450 nm; λem: 560 nm
A5565
(R)-(+)-4-(N,N-Dimethylaminosulfonyl)-7-(2-chloroformylpyrrolidin-1-yl)-2,1,3-benzoxadiazole, HPLC Labeling Reagent for e.e. Determination150993-62-7
(R)-(+)-DBD-Pro-COCl, (R)-(+)-4-(N,N-二甲氨基磺酰基)-7-(2-氯甲酰四氢吡咯-1-基)-2,1,3-苯并恶二唑
λex: 450 nm; λem: 560 nm
A5564
(S)-(-)-4-(N,N-Dimethylaminosulfonyl)-7-(2-chloroformylpyrrolidin-1-yl)-2,1,3-benzoxadiazole, HPLC Labeling Reagent for e.e. Determination150993-63-8
(S)-(-)-DBD-Pro-COCl, (S)-(-)-4-(N,N-二甲氨基磺酰基)-7-(2-氯甲酰四氢吡咯-1-基)-2,1,3-苯并恶二唑
λex: 450 nm; λem: 560 nm
A5578
(S)-(+)-4-(3-Isothiocyanatopyrrolidin-1-yl)-7-nitro-2,1,3-benzoxadiazole, HPLC Labeling Reagent for e.e. Determination163927-30-8
(S)-(+)-NBD-Py-NCS, (S)-(+)-4-(3-异硫氰酸基吡咯烷-1-基)-7-硝基-2,1,3-苯并恶二唑
λex: 488 nm; λem: 590 nm
A5567
(S)-(-)-4-Nitro-7-(2-chloroformylpyrrolidin-1-yl)-2,1,3-benzoxadiazole, HPLC Labeling Reagent for e.e. Determination159717-68-7
(S)-(-)-NBD-Pro-COCl, (S)-(-)-4-硝基-7-(2-氯甲酰四氢吡咯-1-基)-2,1,3-苯并恶二唑
λex: 470 nm; λem: 540 nm
A5566
(R)-(+)-4-Nitro-7-(2-chloroformylpyrrolidin-1-yl)-2,1,3-benzoxadiazole, HPLC Labeling Reagent for e.e. Determination159717-69-8
(R)-(+)-NBD-Pro-COCl, (R)-(+)-4-硝基-7-(2-氯甲酰四氢吡咯-1-基)-2,1,3-苯并恶二唑
λex: 470 nm; λem: 540 nm
A5579
4-(4,5-Diphenyl-1H-imidazol-2-yl)benzoyl Chloride Hydrochloride, for HPLC LabelingN/A
4-(4,5-二苯基-1H-咪唑-2-基)苯甲酰氯盐酸盐
λex: 325 nm; λem: 430 nm
A5558
4-(N,N-Dimethylaminosulfonyl)-7-(N-chloroformylmethyl-N-methylamino)-2,1,3-benzoxadiazole, for HPLC Labeling156153-43-4
DBD-COCl, 4-(N,N-二甲基氨磺酰)-7-(N-氯甲酰甲基-N-甲氨基)-2,1,3-苯并恶二唑
λex: 450 nm; λem: 560 nm
A5595
4-(N,N-Dimethylaminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole, for HPLC Labeling98358-90-8
DBD-F, 4-(N,N-二甲氨基磺酰)-7-氟-2,1,3-苯并恶二唑
λex: 450 nm; λem: 590 nm
A5568
(R)-(-)-4-(N,N-Dimethylaminosulfonyl)-7-(3-isothiocyanatopyrrolidin-1-yl)-2,1,3-benzoxadiazole, for HPLC Labeling163927-31-9
(R)-(-)-DBD-Py-NCS, (R)-(-)-4-(N,N-二甲氨基磺酰基)-7-(3-异硫氰酸基四氢吡咯-1-基)-2,1,3-苯并恶二唑
λex: 460 nm; λem: 550 nm
A5569
(S)-(+)-4-(N,N-Dimethylaminosulfonyl)-7-(3-isothiocyanatopyrrolidin-1-yl)-2,1,3-benzoxadiazole, for HPLC Labeling163927-32-0
(S)-(+)-DBD-Py-NCS, (S)-(+)-4-(N,N-二甲氨基磺酰基)-7-(3-异硫氰酸基四氢吡咯-1-基)-2,1,3-苯并恶二唑
λex: 460 nm; λem: 550 nm
A5592
4-Chloro-7-nitro-2,1,3-benzoxadiazole, for HPLC Labeling10199-89-0
NBD-Cl, 4-氯-7-硝基-2,1,3-苯并恶二唑
λex: 460 nm; λem: 535 nm
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